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Updated: Jun 2, 2026
Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
Published on: June 8, 2012
Determination of internalization of chromium oxide nano-particles in Escherichia coli by flow cytometry
Imrana Khatoon1, Poornima Vajpayee, Gulshan Singh
1Environmental Toxicology Group, Indian Institute of Toxicology Research (CSIR), M. G. Marg, Lucknow 226001, India.
Abstract:
In this study, Escherichia coil DH5alpha (ATCC 35218) were exposed to 0-100 microg/mL chromium oxide nanoparticles (Cr2O3, Nps) for 15-120 min to study the internalization of Nps by flowcytometry. A concentration-duration dependent increased side scatter (SSC) confirmed the internalization of Cr2O3 NPs by the E. coli. This study suggests that the uptake of Nps by bacterial cells can be rapidly monitored with flow cytometry for toxicity and risk assessment.
