Related Experiment Video
Updated: Jun 2, 2026

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Rapid detection of enterotoxigenic Escherichia coli gene using bio-conjugated gold nano-particles
Anurag Jyotil1, Surinder P Singh, Madhu Yashpal
1Environmental Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226001, India.
Abstract:
In this study, a simple gold nanoparticle based colorimetric detection system for on site diagnostics of Enterotoxigenic Escherichia coli (ETEC) has been developed. The developed assay could detect LT1 gene as evident from UV-visible spectra, the broadening of peak on hybridization of GNP probes with different copy numbers of the target gene. The present study suggests that hybridization induced changes in optical properties of GNPs can be translated into a rapid and simple colorometric" spot and read test detection method for ETEC.

