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Structure-based redesign of cofactor binding in putrescine oxidase

Malgorzata M Kopacz1, Stefano Rovida, Esther van Duijn

  • 1Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

Biochemistry
|April 14, 2011
PubMed
Summary

Putrescine oxidase (PuO) structure reveals insights into substrate specificity and cofactor binding. Engineering PuO to form a covalent flavin linkage enhances stability for biocatalysis.

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