Related Experiment Video
Updated: Dec 23, 2025

Assessment of Mitochondrial Oxygen Consumption Using a Plate Reader-based Fluorescent Assay
Published on: April 12, 2024
Mitochondrial consumption of cytosolic ATP: not so fast
1Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary. chinopoulos.christos@eok.sote.hu
Abstract:
In various pathologic circumstances depolarized mitochondria are thought to precipitate cell death by avidly consuming cytosolic ATP. However, for as long as the inner mitochondrial membrane remains intact the reversal potentials of the adenine nucleotide translocase (ANT) and that of F(0)-F(1) ATP synthase are strategically positioned so that they oppose import of cytosolic ATP into the matrix of respiration-impaired mitochondria. This arrangement also seems to protect against a hysteretic consumption of cytosolic ATP accumulating in the mitochondrial matrix, in view of the depolarization caused by inhibition of F(0)-F(1) ATP synthase by the endogenous protein IF1, yielding fast ANT reversal rates.
Related Concept Videos
Chemiosmosis and ATP Synthesis
Mitochondria
Electron Transport Chains
The ETC is comprised of...
Chemiosmosis
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
The ADP/ATP Carrier Protein
The Citric Acid Cycle

