Jove
Visualize
Contact Us

Related Concept Videos

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of a thermogenic target in the dorsal raphe nucleus for weight management in mice.

Nature communications·2026
Same author

Preclinical characterization of MTX-101: a novel bispecific CD8 Treg modulator that restores CD8 Treg functions to suppress pathogenic T cells in autoimmune diseases.

Frontiers in immunology·2024
Same author

Author Correction: Pharmacological targeting of glutamatergic neurons within the brainstem for weight reduction.

Nature metabolism·2022
Same author

Pharmacological targeting of glutamatergic neurons within the brainstem for weight reduction.

Nature metabolism·2022
Same author

The Bispecific Tumor Antigen-Conditional 4-1BB x 5T4 Agonist, ALG.APV-527, Mediates Strong T-Cell Activation and Potent Antitumor Activity in Preclinical Studies.

Molecular cancer therapeutics·2022
Same author

MOR209/ES414, a Novel Bispecific Antibody Targeting PSMA for the Treatment of Metastatic Castration-Resistant Prostate Cancer.

Molecular cancer therapeutics·2016
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 2, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Prostatic acid phosphatase expression in human tissues.

Thomas J Graddis1, Catherine J McMahan, Jennifer Tamman

  • 1Department of Immunology, University of Washington, Seattle, WA, USA.

International Journal of Clinical and Experimental Pathology
|April 14, 2011
PubMed
Summary
This summary is machine-generated.

Prostate cancer immunotherapy shows promise using prostatic acid phosphatase (PAP) as a target antigen. While PAP is found in other tissues, its expression is significantly higher in the prostate, supporting its use in cancer treatment.

Keywords:
Prostatic acid phosphataseimmunohistochemistryimmunotherapyprostate cancerquantitative polymerase chain reaction

More Related Videos

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
06:33

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Published on: July 5, 2021

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Related Experiment Videos

Last Updated: Jun 2, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
06:33

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Published on: July 5, 2021

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Prostate cancer is a leading cause of cancer death in males.
  • Autologous cellular immunotherapy requires specific tumor antigens for efficacy.
  • Prostatic acid phosphatase (PAP) is a potential target antigen due to its prostate expression.

Purpose of the Study:

  • To evaluate the expression profile of Prostatic Acid Phosphatase (PAP).
  • To determine the suitability of PAP as a target antigen for prostate cancer immunotherapy.
  • To validate a multi-method approach for assessing tissue-specific antigen expression.

Main Methods:

  • Immunohistochemistry
  • In situ hybridization
  • Quantitative polymerase chain reaction (qPCR)
  • In silico analysis of human cDNA libraries

Main Results:

  • PAP expression was confirmed in prostate tissues.
  • PAP expression was detected in other human tissues, but at significantly lower levels (1-2 orders of magnitude less).
  • The relative specificity of PAP expression in the prostate was established.

Conclusions:

  • The expression profile of PAP supports its use as a target antigen for prostate cancer immunotherapy.
  • A comprehensive approach using multiple expression correlates is crucial for selecting immunotherapy targets.
  • This study validates a methodology for identifying suitable antigens for cellular immunotherapy.