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Updated: Jun 2, 2026

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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
Published on: March 3, 2015
Functional phosphosite screening for targeted protein-protein interactions by combining phosphoproteomics strategies
Mingquan Guo1, Zanxian Xia, Hong Ma
1Keck School of Medicine, University of Southern California, 1501 San Pablo St., Los Angeles, CA 90033, USA. mingquag@usc.edu.
Molecular Biosystems
|April 14, 2011
Abstract:
An effective new platform for phosphosite mapping and subsequent functional screening was developed to analyze the targeted protein-protein interactions of p300 and CBP with β-catenin. Two novel functional phosphosites, Ser12 of p300 and Ser92 of CBP, were revealed to modulate p300/β-catenin and CBP/β-catenin interactions, respectively.
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