Related Experiment Videos
[Effects of plasminogen activator on epidermal cell migration]
1Department of Dermatology, Juntendo University School of Medicine.
Nihon Hifuka Gakkai Zasshi. the Japanese Journal of Dermatology
|October 1, 1990
Summary
The plasminogen activator/plasmin system significantly influences keratinocyte migration. Inhibiting serine proteinases or urokinase suppressed migration, while adding plasminogen enhanced it.
Area of Science:
- Cell biology
- Biochemistry
- Dermatology
Background:
- Keratinocyte migration is crucial for wound healing and skin repair.
- The role of the plasminogen activator/plasmin system in this process requires further elucidation.
Purpose of the Study:
- To investigate the involvement of plasminogen activator (PA) and plasmin in regulating keratinocyte migration.
- To determine the effect of specific proteinase inhibitors and plasminogen on keratinocyte motility.
Main Methods:
- Utilized a wound healing model in cultured keratinocytes.
- Assessed the impact of various proteinase inhibitors, including camostat mesilate and anti-urokinase IgG, on cell migration.
- Evaluated the effect of exogenous plasminogen on keratinocyte migration rates.
Main Results:
- Camostat mesilate, a serine proteinase inhibitor, demonstrated a concentration-dependent inhibition of keratinocyte migration.
- Anti-urokinase IgG significantly suppressed keratinocyte migration.
- Exogenous plasminogen addition led to enhanced keratinocyte migration.
Conclusions:
- The findings strongly indicate that the plasminogen activator/plasmin system plays a significant role in keratinocyte migration.
- This system represents a potential therapeutic target for modulating skin repair processes.