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Updated: Jun 2, 2026
Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
Published on: October 9, 2010
Extraneuronal toxicity of Alzheimer's β-amyloid peptide: comparative study on vertebrate skeletal muscles
Marat A Mukhamedyarov1, Alexander Y Teplov, Sergey N Grishin
1Department of Physiology, Kazan State Medical University, ul. Butlerova 49, R-420012 Kazan, Russia. maratm80@list.ru
Introduction:
Alzheimer's β-amyloid peptide (βAP) is known to possess a wide range of toxic effects on neurons in vitro and in vivo; however, there is little information available regarding its impact on other excitable tissues such as skeletal muscles, which, apart from brain cells, are thought to also be targets of βAP.
Methods:
Utilizing the combination of electrophysiology and myography, we investigated whether βAP also impairs the functioning of myocytes in frogs and mice.
Results:
Although application of βAP in the range of 10(-6) to 10(-8) M induced depolarization of muscle fibers in both species, it impaired contractility in frogs but not in mice, by reducing endplate potential amplitude and increasing the threshold potential.
Conclusions:
Unchanged contractility in the mouse in the presence of βAP is due to a higher safety factor of neuromuscular transmission in mammals compared with amphibians. Possible clinical implications are discussed.
