Related Experiment Video
Updated: Jun 2, 2026

Extracellular Vesicle Tissue Factor Activity Assay
Published on: December 29, 2023
[Effect of tissue factor in extravascular migration of fibrosarcoma cells]
Chun-Shan Han1, Hua Xin, Zhen-Guo Han
1Department of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
Objective:
To evaluate the effect of tissue factor (TF) in extravascular migration of fibrosarcoma cells and hematogenous metastasis.
Methods:
The expression of tissue factor in fibrosarcoma HT1080 cells was analyzed by flow cytometry. The extravascular migration of fibrosarcoma cells was observed in a constructed monolayer vascular endothelial cells and extra-cellular matrix model.
Result:
Tissue factor was highly expressed in HT1080 cells. HT1080 migrated and passed through the monolayer vascular endothelial cells to the collagen gel in a time-dependent manner. Anti-TF antibody inhibited extravascular migration of fibrosarcoma cells and the inhibition was concentration-dependent (P<0.05).
Conclusion:
Tissue factor may enhance hematogenous metastasis through extravascular migration of fibrosarcoma cells.
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