Related Experiment Video
Updated: Jun 2, 2026
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
Published on: October 20, 2011
Confocal laser microscopic analysis of biofilm on newer feldspar ceramic
A S Brentel1, K Z Kantorski, L F Valandro
1Department of Dental Materials and Prosthodontics, São Paulo State University, São José dos Campos, Brazil.
Purpose:
This study evaluated the surface roughness, hydrophobicity and in situ dental biofilm associated with microfilled feldspar ceramics submitted to the different finishing and polishing procedures.
Methods And Materials:
Samples were made according to the manufacturer's instructions and allocated to groups as follows: glaze (G1); glaze and diamond bur (G2); glaze, diamond bur and rubber tips (G3) and glaze, diamond bur, rubber tips and felt disks impregnated with a fine-aluminum oxide particle based paste (G4). Roughness was evaluated with a roughness analyzer (Ra). Hydrophobicity was determined by the contact angle of deionized water on samples. Biofilm was evaluated eight hours after formation in the oral environment using confocal laser-scanning microscopy (CLSM) and scanning-electron microscopy (SEM).
Results:
Significant differences were found related to roughness (G1
Conclusion:
Polishing with rubber points followed by felt disks impregnated with a fine-aluminum oxide particle resulted in biofilm formation similar to that present with a glazed ceramic surface, even though the surface is still rougher and more hydrophobic.
