Lipoprotein enrichment in orange insoluble particulate matter reproducibly appearing in cryoprecipitate
W P Sheffield1, V Bhakta, B Trigatti
1Canadian Blood Services Research and Development, and Departments of Pathology andMolecular Medicine, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada. sheffiel@mcmaster.ca
Background And Objective:
Cryoprecipitate prepared from two whole blood donations from the same donor contained insoluble orange particulate material (OPM). We sought to identify the OPM.
Materials And Methods:
OPM was recovered from the blood product by centrifugation, dissolved in sodium dodecyl sulphate (SDS) and analysed by SDS-polyacrylamide gel electrophoresis and immunoblotting.
Results:
Solubilized OPM was enriched in apolipoproteins B and E, but not apolipoprotein A1, immunoglobulin G or albumin, suggesting lipoprotein enrichment in OPM. Subsequent clinical laboratory blood tests confirmed low-density lipoprotein hyperlipidaemia with normal triglyceride levels. Further, cryoprecipitate production from this donor was prevented by implementation of national predominantly male plasma policies.
Conclusion:
Cryoprecipitate produced from hyperlipidaemic donors may contain insoluble particles that render it inappropriate for transfusion.
