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Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
Published on: January 31, 2020
The use of dynamic surface chemistries to control msc isolation and function.
J M Curran1, F Pu, R Chen
1UKCTE, Clinical Engineering, The Institute of Ageing and Chronic Disease, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, United Kingdom. j.curran@liv.ac.uk
Biomaterials
|April 15, 2011
Summary
Optimized methyl (-CH(3)) surface modifications enhance mesenchymal stem cell (MSC) adhesion and isolation. Specific chain lengths, like ODMCS, significantly boost MSC markers and cell expansion potential.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Surface Chemistry
Background:
- Material surface modifications, particularly with methyl (-CH(3)) and amine (-NH(2)) groups, can influence mesenchymal stem cell (MSC) behavior.
- Current methods for presenting these chemical groups on surfaces lack controlled presentation, leading to variable MSC adhesion and differentiation outcomes.
Purpose of the Study:
- To define the potential of optimized dynamic methyl (-CH(3)) surface chemistry for controlling initial MSC adhesion, integrin binding, and subsequent cell function.
- To investigate the impact of varying -CH(3) chain lengths and bonding mechanisms on MSC support.
Main Methods:
- Fabrication of glass substrates modified with various -CH(3) silane chain lengths and bonding mechanisms.
- Assessment of adult MSC adhesion, viability, and expression of key markers (STRO-1, CD29, CD73, CD90, CD105).
- Analysis of Fibroblast Growth Factor (FGF) release and its correlation with MSC response.
Main Results:
- Surface modification with chlorodimethyloctylsilane (ODMCS) significantly increased adult MSC marker expression compared to other -CH(3) surfaces and controls.
- Dichlorodimethylsilane (DMDCS) modified surfaces inhibited MSC adhesion due to excessive early FGF release but improved MSC isolation from whole blood.
- Surface chemistry influenced MSC adhesion, FGF release, and marker expression, with ODMCS showing superior performance for MSC support.
Conclusions:
- Optimized -CH(3) surface modifications offer a cost-effective method for enhancing MSC isolation and expansion.
- These tailored surfaces show potential to replace traditional modifications like RGD and fibronectin in specific applications.
- Controlled surface chemistry is crucial for manipulating MSC adhesion and function, with implications for regenerative medicine.
