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Related Concept Videos

Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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Related Experiment Video

Updated: Jun 2, 2026

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

PGD for reciprocal and Robertsonian translocations using array comparative genomic hybridization.

F Fiorentino1, L Spizzichino, S Bono

  • 1GENOMA-Molecular Genetics Laboratory, Rome, Italy. fiorentino@laboratoriogenoma.it

Human Reproduction (Oxford, England)
|April 15, 2011
PubMed
Summary
This summary is machine-generated.

Array comparative genomic hybridization (array-CGH) offers a molecular-based approach for preimplantation genetic diagnosis, improving detection of embryonic chromosome abnormalities. This method enhances accuracy and reliability over traditional FISH techniques.

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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

FISH for Pre-implantation Genetic Diagnosis
07:34

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

Related Experiment Videos

Last Updated: Jun 2, 2026

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

FISH for Pre-implantation Genetic Diagnosis
07:34

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

Area of Science:

  • Reproductive Medicine
  • Genetics
  • Embryology

Background:

  • Fluorescence in situ hybridization (FISH) is standard for detecting embryonic chromosome issues but has limitations.
  • Array comparative genomic hybridization (array-CGH) is a molecular assay for screening embryos.

Purpose of the Study:

  • To describe the clinical application of array-CGH for preimplantation genetic diagnosis.
  • To simultaneously screen for aneuploidy and unbalanced translocation derivatives in all 24 chromosomes of embryos.

Main Methods:

  • Cleavage-stage embryo biopsy (Day 3) followed by whole-genome amplification (WGA).
  • Array-CGH analysis using 24sure+ arrays.
  • Selection of euploid embryos for transfer on Day 5.

Main Results:

  • 28 cycles of preimplantation genetic diagnosis were performed for 24 couples with balanced translocations.
  • 93.5% of embryos (187/200) were successfully diagnosed.
  • Clinical pregnancy was achieved in 70.6% of embryo transfers, with ongoing pregnancies and three healthy births.

Conclusions:

  • Array-CGH effectively detects embryonic chromosome imbalances and aneuploidy.
  • This method overcomes FISH limitations, offering improved performance, automation, sensitivity, and reliability.