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Related Experiment Video

Updated: Jun 2, 2026

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
08:32

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Published on: March 24, 2011

Imaging C. elegans embryos using an epifluorescent microscope and open source software.

Koen J C Verbrugghe1, Raymond C Chan

  • 1Human Genetics, University of Michigan, USA.

Journal of Visualized Experiments : Jove
|April 15, 2011
PubMed
Summary

Live-cell imaging of dynamic cellular processes in Caenorhabditis elegans embryos is now accessible. This protocol details time-lapse microscopy methods using standard equipment for studying development and cell biology in vivo.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Microscopy

Background:

  • Cellular processes like chromosome segregation are dynamic and best studied in live cells.
  • Time-lapse imaging in intact organisms is crucial for understanding developmental regulation.
  • The Caenorhabditis elegans embryo is an ideal model due to its transparency, rapid development, and known cell lineage.

Purpose of the Study:

  • To present methods for live imaging of Caenorhabditis elegans embryos.
  • To demonstrate the utility of standard epifluorescent microscopes for time-lapse analysis.
  • To highlight the use of open-source software for automating imaging.

Main Methods:

  • Utilizing differential interference contrast (DIC) microscopy.
  • Employing green fluorescent protein (GFP) fluorescence imaging.
  • Adapting standard compound epifluorescent microscopes for time-lapse studies.
  • Using open-source software for image acquisition automation.

Main Results:

  • Established protocols for live imaging of C. elegans embryos.
  • Demonstrated that readily available microscopes can perform time-lapse analysis.
  • Showcased the ease of studying dynamic cellular and developmental processes in vivo.

Conclusions:

  • Live imaging of C. elegans embryos is feasible with standard equipment.
  • This approach facilitates the study of fundamental cellular and developmental processes.
  • The methods presented enable in vivo investigation of dynamic biological events.