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Determination of aflatoxin B1 in cereals by homogeneous liquid-liquid extraction coupled to high performance liquid
Neda Sheijooni-Fumani1, Jalal Hassan, Seyed R Yousefi
1International Goods Inspection Company, Tehran, Iran. n.sheijooni@yahoo.com
Abstract:
A simple and rapid method based on homogeneous liquid-liquid extraction coupled to HPLC with fluorescence detection was developed for the determination of aflatoxin B1 (AFB1) in the rice and grain samples after post-column derivatization. The proposed method eliminated the use of immunoaffinity columns for clean-up in the determination of AFB1. The parameters affecting recovery and preconcentration such as type and volume of organic solvent, volume ratio of water/methanol, concentration of phase separator reagent and extraction time were optimized. Under the optimized conditions, the calibration graph was linear in the concentration range of 0.01-1.0 ng/g with the detection limit of 0.003 ng/g. This method was successfully applied for the analysis of AFB1 in different cereal samples.
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