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Total antioxidant status in the blood serum of rats after biogenic amines administration
Nina Zamosteanu1, Cristiana Filip, Elena Albu
1School of Medicine, Department of Biochemistry, Gr.T. Popa University of Medicine and Pharmacy Iaşi.
Unlabelled:
The aim of our study was to investigate the influence of biogenic amines on rat blood levels of total antioxidant status (TAS). Total antioxidant species refers to all circulating species in plasma including vitamin E, vitamin C, beta-carotene, uric acid, bilirubin, albumin as well as metal-binding proteins (e.g. ferritin or ceruloplasmin).
Material And Method:
We worked on three series of Wistar male rats. Series I received histamine (10 mg/kg body) intra peritoneal (i.p.) single dose, Series II received tyramine (10 mg/kg body) intra peritoneal (i.p.) single dose and Series III received biogenic amines mixture (histamine, tyramine and cadaverine 5 mg/kg body) intra peritoneal (i.p.) single dose. At 72 hours after biogenic amines administration blood samples were collected and total antioxidant status was determined using a RANDOX kit for manual use.
Results:
Our data shows that total antioxidant status present a significant decrease after 72 hours after amines administration as compared with initial moment, before administration.
Conclusions:
The biogenic amines decrease the level of the total oxidant status in rat blood serum and reduce the capacity of the antioxidant defense system. The amines administration as a mixture seems to have no cumulative effect on individual amine toxicity.