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Related Experiment Video

Updated: Jun 2, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantitative label-free phosphoproteomics strategy for multifaceted experimental designs.

Erik J Soderblom1, Melanie Philipp, J Will Thompson

  • 1Proteomics Core Facility, Institute for Genome Science & Policy, Duke University Medical Center, Durham, North Carolina 27710, USA.

Analytical Chemistry
|April 16, 2011
PubMed
Summary

This study introduces a new label-free phosphoproteomic method for analyzing small biological samples. This advance enables quantitative phosphorylation analysis in diverse tissues and fluids, aiding disease research.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Cellular Biology

Background:

  • Protein phosphorylation is vital for eukaryotic cell signaling.
  • Dysregulated phosphorylation is linked to various diseases.
  • Current quantitative phosphoproteomics often requires large sample amounts from cell lines.

Purpose of the Study:

  • To develop a general label-free quantitative phosphoproteomic strategy.
  • To enable analysis of small biological samples, including human tissues and fluids.
  • To overcome limitations of current methods requiring large starting material quantities.

Main Methods:

  • Utilized a titanium dioxide (TiO2) enrichment protocol.
  • Optimized phosphopeptide selectivity and recovery using a condition matrix.

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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Related Experiment Videos

Last Updated: Jun 2, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

  • Validated quantitative reproducibility (16% RSD) and accuracy (within 5% of theoretical values).

    • Main Results:

    • Successfully quantified 719 phosphorylated peptides from 449 proteins.
    • Analyzed samples from 200 μg of zebrafish embryo lysates.
    • Demonstrated application in a GRK5 knockdown model in zebrafish embryos.

    Conclusions:

    • The developed TiO2 enrichment strategy is effective for label-free quantitative phosphoproteomics.
    • The method is applicable to small biological samples, including human tissues and fluids.
    • This approach facilitates phosphoproteomic studies in disease-related research with limited sample availability.