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The fluorescence theatre: a cost-effective device using theatre gels for fluorescent protein and dye screening

John R Heil1, Ricardo F Nordeste, Trevor C Charles

  • 1Department of Biology, University of Waterloo, ON, Canada.

Canadian Journal of Microbiology
|April 16, 2011
PubMed

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...