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Updated: Jun 2, 2026
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities
Published on: February 20, 2021
Effect of X-irradiation on the quality of red cell concentrates
1NHS Blood and Transplant, Brentwood, Essex, UK. saber.bashir@nhsbt.nhs.uk
Introduction:
The irradiation of blood components is used to inactivate T-lymphocytes to prevent transfusion-associated graft-versus-host (GVH) disease in susceptible recipients. X-irradiation can be used as an alternative to gamma irradiation and does not involve the use of a radioactive source. This study investigated the effect of X-irradiation on the quality of various red cell concentrates (RCC).
Methods:
Whole blood units were processed into RCC in additive solution (SAG-M) or RCC in plasma suitable for intra-uterine (IUT) or neonatal exchange transfusion. RCC-SAG-M were irradiated at day 14 and sampled before and 0, 7 and 14 days following irradiation. RCC-IUT and RCC-Exchange were irradiated on day 4 and sampled before and 0 and 24 h following irradiation. Extracellular potassium levels and free plasma haemoglobin (haemolysis) were compared in X or gamma-irradiated units during storage.
Results:
X-irradiation of RCC in SAG-M, or RCC for IUT or Exchange resulted in haemolysis comparable with gamma irradiation and levels were below the current UK/CE limit of 0.8% at the end of shelf life. X-irradiation of RCC-SAG-M or RCC-IUT with haematocrit of 75% and 85% resulted in potassium leakage comparable with gamma-irradiation. However, X-irradiation of RCC for neonatal exchange transfusion showed statistically significantly higher supernatant potassium levels after 24 h than gamma irradiation, although this difference is considered to be clinically insignificant.
Conclusion:
X-irradiation of RCC in SAG-M or IUT or RCC in plasma suitable for neonatal exchange transfusion resulted in acceptable levels of haemolysis and potassium leakage compared with the current process of gamma irradiation.
