Oxidatively generated damage to DNA by UVA radiation in cells and human skin
1Laboratoire Lésions de Acides Nucléiques, SCIB-UMR-E no 3, CEA/UJF, FRE CNRS 3200, Département de Recherche Fondamentale sur la Matière Condensée, Grenoble, France. jean.cadet@cea.fr
Abstract:
Exposure of cells and human skin to UVA radiation oxidatively induces damage to DNA that consists mostly of 8-oxo-7,8-dihydroguanine (8-oxoGua), together with relatively minor amounts of oxidized pyrimidine bases and oligonucleotide strand breaks. Singlet oxygen generated by the type II photosensitization mechanism forms 8-oxoGua, with a small contribution by hydroxyl radicals. The mechanisms of UVA-mediated formation of DNA oxidation products indicate the absence of induction of double-strand breaks.
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