Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

Peptide-modified surfaces for enzyme immobilization.

Jinglin Fu¹, Jeremy Reinhold, Neal W Woodbury

¹Center for Single Molecule Biophysics, Arizona State University, Tempe, Arizona, United States of America.

|April 16, 2011

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From randomness to recognition: modeling the evolution of DNA sequence information during enrichment for binding.

Bioinformatics advances·2026

Same author

Incorporation of NUPACK-Based Simulation into Classroom and Laboratory Teaching of Nucleic Acids Hybridization for Undergraduate Biochemistry.

Journal of chemical education·2025

Same author

Analyzing DNA Origami Nanostructure Assembly by Dynamic Light Scattering and Nanoparticle Tracking Analysis.

Small methods·2025

Same author

A percolation phase transition controls complement protein coating of surfaces.

Cell·2025

Same author

Rapid Amplification and Detection of Single-Stranded Nucleic Acids for Point-of-Care Diagnosis.

Small methods·2025

Same author

A percolation-type criticality threshold controls immune protein coating of surfaces.

bioRxiv : the preprint server for biology·2024

Same journal

Invaders taking over-Mollusc faunal change in volcanic barrier lakes of the Albertine Rift biodiversity hotspot.

PloS one·2026

Same journal

AI-driven molecular diversification and ligand-based optimization of macitentan derivatives targeting VEGFR1 and endothelin signaling pathways.

PloS one·2026

Same journal

Performance patterns and records in the world aquatics masters championships: Where do the most frequently represented nations among the top-ten masters swimmers come from?

PloS one·2026

Same journal

Modeling diurnal Temperature-Rainfall relationships under multicollinearity using PLS-SEM: A case study of Ghana.

PloS one·2026

Same journal

Organizational culture, social capital, and emergency capacity in primary healthcare institutions: A cross-sectional structural equation modeling study comparing ordinary and older communities.

PloS one·2026

Same journal

Impact of kidney function on the metabolome in the general population.

PloS one·2026

See all related articles

Researchers developed a novel peptide-based method for enzyme immobilization on surfaces. This approach enhances enzyme activity, stability, and orientation control, offering a versatile tool for biocatalysis and surface chemistry applications.

Area of Science:

Surface chemistry
Enzymology
Biocatalysis

Background:

Enzymology at surfaces is crucial for biological systems and biocatalysis.
Current enzyme immobilization methods struggle with controlling orientation, reducing nonspecific binding, and preventing denaturation.
Advanced strategies are needed for precise control over enzyme orientation and position to optimize activity.

Purpose of the Study:

To develop a novel method for enzyme immobilization using peptide ligands.
To improve enzyme activity, stability, and orientation control on surfaces.
To demonstrate the efficacy of peptide-modified surfaces for enzyme capture and performance.

Main Methods:

Selection of peptide ligands from high-density arrays for enzyme binding.

More Related Videos

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Published on: April 11, 2020

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

Related Experiment Videos

Last Updated: Jun 2, 2026

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Published on: April 11, 2020

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

Optimization of peptide sequences via single-point variant screening to enhance affinity and activity.

Covalent attachment of selected peptides to surfaces for enzyme immobilization.

Main Results:

Peptide ligands were identified that bind and enhance the activity of β-galactosidase.
Enzymes immobilized on peptide-modified surfaces showed higher specific activity and stability compared to conventional methods.
Controlled protein orientation was achieved using the peptide immobilization strategy.

Conclusions:

A straightforward method for enzyme immobilization via specific peptide-surface interactions has been established.
This technique offers broad applicability for immobilizing diverse enzymes with optimized performance.
The method enables patterned self-assembly of multiple enzymes on surfaces.