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Related Experiment Video

Updated: Jun 2, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Diaphragm pico-liter pump for single-cell manipulation.

Yasser Anis1, Jeffrey Houkal, Mark Holl

  • 1Mechanical Design and Production Engineering, Cairo University, Giza, 12316, Egypt. yasser.anis@ieee.org

Biomedical Microdevices
|April 16, 2011
PubMed
Summary
This summary is machine-generated.

A novel pico-liter pump integrated into a robotic system precisely handles individual living cells. This automated cell manipulation technology minimizes stress on cells during transfer for analysis.

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Last Updated: Jun 2, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
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Published on: December 6, 2013

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Robotics

Background:

  • Automated handling of individual living cells is crucial for various biological analyses.
  • Existing methods for cell transfer can cause significant stress and perturbation to delicate biological samples.

Purpose of the Study:

  • To develop and integrate a pico-liter pump into a robotic system for automated, precise selection and transfer of single living cells.
  • To minimize cell perturbation during aspiration and dispensing by controlling fluid dynamics.

Main Methods:

  • Development of a displacement-type pico-liter pump with a piezoelectric actuator-driven diaphragm.
  • Integration of the pump into a robotic manipulation system for automated cell selection and transfer.
  • Characterization of fluid volumes (500 pL to 250 nL) and flow rates (up to 250 nL/s).

Main Results:

  • The developed pump successfully aspirates and dispenses pico-liter to nano-liter volumes.
  • The piezo-driven diaphragm enables precise control over flow rates and fluid dynamics.
  • Minimized shear stresses were achieved, reducing perturbation to live cells.

Conclusions:

  • The integrated robotic system with a pico-liter pump offers a highly precise and gentle method for single living cell manipulation.
  • This technology has significant potential for advancing high-throughput biological assays and single-cell analysis.
  • The system's ability to control shear stress is key for preserving cell viability and function.