Synergistic interactions between Glomus mosseae and Bradyrhizobium japonicum in enhancing proton release from nodules and hyphae
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Updated: Jun 2, 2026

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
Published on: March 14, 2025
Xiaodong Ding1, Xinhua Sui, Fang Wang
1Key Laboratory of Plant-Soil Interactions, Ministry of Education, College of Resource and Environmental Science, China Agricultural University, Beijing, China.
Abstract:
Soybean (Glycine max L. Merr.) seedlings were inoculated with Glomus mosseae (GM) and Bradyrhizobium japonicum (BJ) together or separately to study the effect of interactions on net H(+) effluxes of nodules or extraradical hyphae by in vivo vibrating electrode techniques. GM promoted three-fold the H(+) effluxes of nodules on mycorrhizal lateral roots and BJ increased eight-fold the net H(+) effluxes of hyphae developing in the vicinity of nodules on lateral roots. Increments in plant P content were positively and linearly correlated with the net H(+) efflux of nodules and hyphae. It is concluded that increased H(+) effluxes of nodules resulted from enhanced nitrogenase activities induced by the presence of the AM fungus in lateral roots. The results point to additive effects of interactions between mycorrhizal fungi and rhizobia in increasing the extent of acidification of the "nodulesphere" and the hyposphere.
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