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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
Published on: May 21, 2019
Analysis of microRNA expression profiling identifies microRNA-503 regulates metastatic function in hepatocellular
1Department of Stomatolog, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Background And Objectives:
Metastasis of cancer is a complex process that involves multiple alterations. Recent evidence indicates that small non-protein coding RNA molecules (miRNAs) might be involved in cancer-related processes in humans. This study was to systematically investigate the differentially expressed miRNAs during metastasis in hepatocellular carcinoma (HCC) using microarray technology.
Methods:
The differentially expressed miRNAs between HCCLM3 and MHCC97-L, two HCC cell lines with differently metastatic potentials were displayed using microarray technology. The expression of miR-503 was verified by the real-time quantitative polymerase chain reaction. In addition, the lentivirus-delivered system for expressing miR-503 in HCCLM3 cells was employed to investigate whether miR-503 was involved in invasive phenotype of HCC cell.
Results:
Our study built a metastasis-related miRNAs expression profiling, which includes 327 miRNAs expressed differentially between HCCLM3 and MHCC97-L cell lines. Furthermore, expression of miR-503 by lentivirus-delivered system in HCCLM3 cell was established successfully. Our results showed that miR-503 induces a G1 arrest and decreased proliferation for HCCLM3 cell (P < 0.05). In addition, miR-503 inhibits migration and invasion of HCCLM3 cell in vitro (P < 0.05).
Conclusions:
This study described a metastasis-related miRNAs expression profiling and revealed miR-503 regulating metastatic function in HCC cell.
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