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Updated: Jun 2, 2026

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
Published on: November 26, 2018
Time-varying migration process of moving neutralization boundary on the immobilized pH gradient strip in the
Heng Liang1, Liang-Fei OuYang, Qian Liu
1Separation Science Institute, Key Laboratory of Biomedical Information Engineering of Education Ministry, Xi'an Jiaotong University, Xi'an, P. R. China. lheng@mail.xjtu.edu.cn
Abstract:
This paper quantificationally probes into time-varying migration processes of moving neutralization boundary (MNB) on immobilized pH gradient (IPG) strip in ammonia-rehydration buffers. The time-varying migration processes are determined by both time-varying dissociation equilibria of ammonia and position-varying pH environments formed by immobilized carrier ampholytes (CAs) on the IPG strip. Thus, the local dissociation equilibria of ammonia and the position-varying pH are introduced into the recursion equation of position of MNB migrations. The theoretical position-time curves and the velocity-time curves of MNB migrations obtained by the recursion approach were satisfactorily validated by a series of images of boundary migrations from the IPG-MNB experiments by using rehydration buffers with different ammonia concentrations on pH 3-6 IPG strips. The results achieved herein have significant evidence to a quantificational understanding of the mechanism of MNB and IEF.
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