Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
Protein Folding01:22

Protein Folding

Overview
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NMR-based serum metabolomic signatures distinguish active tuberculosis from latent tuberculosis infection.

Biophysical chemistry·2026
Same author

Comparative toxicity of nickel titanate and calcium manganite perovskite nanomaterials in human and bacterial systems: Implications for environmental and health risks.

Chemosphere·2026
Same author

Rapid Microwave-Assisted Green Synthesis of N,S Co-Doped Red-Emissive Carbon Dots From Coconut Fiber Waste for Label-Free Ultrasensitive Creatinine Detection.

Chemistry, an Asian journal·2026
Same author

From Scaffold to Therapy: Benzimidazole Pharmacophore Evolution in Breast Cancer Research.

Chemistry & biodiversity·2026
Same author

Rapid, point-of-care, visual detection of RBD of SARS-CoV-2 spike protein using an acrylamide-free photonic crystal aptasensor.

RSC advances·2026
Same author

Chromatographic Estimation of Curcumin and Parthenolide Using a Validated RP-HPLC Method.

Current drug research reviews·2026

Related Experiment Video

Updated: Jun 2, 2026

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

Large-area protein patterns generated by ordered binary colloidal assemblies as templates.

Gurvinder Singh1, Vipul Gohri, Saju Pillai

  • 1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Ny Munkegade, Building 1521, 8000 Aarhus C, Denmark.

ACS Nano
|April 19, 2011
PubMed
Summary

Binary colloidal assemblies create tunable gold patterns for selective protein adsorption. This novel lithography method allows adjustable pattern size and spacing for advanced biomaterial applications.

More Related Videos

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

Related Experiment Videos

Last Updated: Jun 2, 2026

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

Area of Science:

  • Materials Science
  • Nanotechnology
  • Surface Chemistry

Background:

  • Precise control over surface chemistry and topography is crucial for biomaterial development.
  • Existing lithography techniques face limitations in achieving tunable nanoscale patterns efficiently.

Purpose of the Study:

  • To develop a versatile lithographic method using binary colloidal assemblies for creating tunable gold patterns.
  • To demonstrate the modification of these patterns for selective protein adsorption.

Main Methods:

  • Utilizing binary colloidal assemblies (large and small particles) as lithographic masks.
  • Self-assembly of particles from dilute dispersions with varying size ratios (0.10–0.50).
  • Surface modification of gold or silicon dioxide regions with protein-resistant molecules.

Main Results:

  • Generation of tunable gold patterns on silicon dioxide substrates with dimensions from micrometers to nanometers.
  • Demonstration of adjustable pattern size and spacing by varying particle choice.
  • Achieved site-selective protein adsorption using surface-modified patterns.

Conclusions:

  • Binary colloidal assemblies offer a flexible platform for nanoscale lithography.
  • The developed method enables the creation of chemically patterned surfaces for controlled biomolecular interactions.
  • This approach facilitates the design of advanced biosensors and functionalized surfaces.