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Updated: Jun 2, 2026
Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
A hybrid approach to direct pulp capping by using emdogain with a capping material
Khalid Al-Hezaimi1, Badr Abdullah Al-Tayar, Yaseer Salim Bajuaifer
1College of Dentistry, King Saud University, Riyadh, Saudi Arabia. hezaimik16@gmail.com
Introduction:
This study evaluated the formation of reparative hard tissues in baboon pulps after Emdogain (EMD) application in conjunction with 3 pulp-capping materials.
Methods:
Thirty-two premolars in four 3-year-old baboons were randomly assigned to 1 of 3 pulp-capping materials. A split-mouth design and intra-animal side randomization were applied to 3 experimental groups (calcium hydroxide, ProRoot White mineral trioxide aggregate, white Portland cement) and the control group (no pulp-capping material). In the hybrid EMD approach, a small drop of EMD was placed over the exposure site after arrest of hemorrhage. The designated pulp-capping material was placed over the EMD, followed by placement of resin-modified glass ionomer cement over the set/unset pulp-capping material. The animals were killed after 4 months. Histomorphometric analysis and micro-computed tomography were performed on the retrieved specimens.
Results:
All groups capped with EMD and 1 of the 3 capping materials exhibited similar reparative tissue thickness (P > .05). Dentin tunnel defects were absent in the mineral trioxide aggregate and Portland cement groups after the use of EMD. However, only a tubular was identified from all specimens.
Conclusions:
Mineral trioxide aggregate produces a better quality reparative hard tissue response with the adjunctive use of Emdogain, when compared with the use of calcium hydroxide.
