Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Does lateral specialization apply to the frog's brain? An electron microscope observation.

M Kemali, V Guglielmotti, D Gioffré

    Zeitschrift Fur Mikroskopisch-Anatomische Forschung
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diamond layers grown by chemical vapor deposition on NbN systems and NbN/SiO2-based devices.

    Journal of nanoscience and nanotechnology·2011
    Same author

    Low temperature conductivity of carbon nanotube aggregates.

    Journal of physics. Condensed matter : an Institute of Physics journal·2011
    Same author

    Polycrystalline diamond on self-assembled detonation nanodiamond: a viable route for fabrication of all-diamond preformed microcomponents.

    Nanotechnology·2011
    Same author

    The dense core of vertebrate central nervous system synapses revealed by potassium permanganate fixation as formed by small membrane-bounded vesicles.

    Neuroscience letters·2009
    Same author

    The ultrastructure of the large granular vesicles of the frog's habenula.

    Neuroscience letters·2009
    Same author

    An innovative and viable route for the realization of ultra-thin supercapacitors electrodes assembled with carbon nanotubes.

    Journal of nanoscience and nanotechnology·2009

    Researchers studied crystal-like inclusions in frog brain cells. These unique structures were found only in a specific part of the left habenula in adult frogs and tadpoles.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Electron Microscopy

    Background:

    • The habenula is a brain region involved in processing reward and aversion.
    • Polymorphic crystal-like inclusions have been observed in neuronal cytoplasm.
    • Previous research has not fully characterized these inclusions in Rana esculenta.

    Purpose of the Study:

    • To investigate the ultrastructure of polymorphic crystal-like inclusions in the frog habenula.
    • To determine the precise location and morphology of these inclusions.
    • To evaluate the effect of different fixatives on the preservation of inclusion ultrastructure.

    Main Methods:

    • Transmission electron microscopy was used to examine neuronal cytoplasm.
    • Three fixatives were employed: osmium tetroxide, a mixture of aldehydes, and potassium permanganate.

    Related Experiment Videos

  • The study included both adult Rana esculenta frogs and their tadpoles.

    • Main Results:

    • Polymorphic crystal-like inclusions were exclusively identified in the cytoplasm of neurons within the medial portion of the left habenula.
    • The inclusions exhibited distinct ultrastructural features dependent on the fixative used.
    • No such inclusions were observed in other brain regions or in glial cells.

    Conclusions:

    • The medial portion of the left habenula in Rana esculenta harbors unique, neuron-specific crystal-like inclusions.
    • The observed ultrastructure is influenced by fixation methods, highlighting the importance of technique in electron microscopy.
    • These findings contribute to our understanding of neuronal heterogeneity and intracellular structures in amphibians.