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Related Experiment Videos

Scatter factor regulates vascular endothelial cell motility.

E M Rosen1, W Carley, I D Goldberg

  • 1Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510.

Cancer Investigation
|January 1, 1990
PubMed
Summary

Scatter factors (SFs) are cytokines that promote cell movement. These factors, derived from mesenchymal cells, significantly enhance vascular endothelial cell migration, suggesting roles in tissue repair and development.

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Area of Science:

  • Cell biology
  • Biochemistry
  • Developmental biology

Background:

  • Mesenchymal cell-derived cytokines, known as scatter factors (SFs), are implicated in stimulating epithelial cell motility.
  • Understanding the specific functions of SFs in different cell types is crucial for regenerative medicine and developmental studies.

Purpose of the Study:

  • To investigate the effect of scatter factors (SFs) on vascular endothelial cell migration.
  • To explore the differential response of endothelial cells based on their morphology to SFs.

Main Methods:

  • Purification of scatter factors (SFs) from ras-transformed 3T3 cell supernatants.
  • Assaying vascular endothelial cell migration in response to purified SFs at low concentrations (picomolar range).
  • Comparing the migratory response of endothelial cell lines with distinct morphologies (cobblestone vs. elongated).

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Main Results:

  • Purified SFs markedly stimulated vascular endothelial cell migration at concentrations below 100 pM.
  • Endothelial cell lines exhibiting a cobblestone (epithelioid) morphology responded to SFs.
  • Endothelial cell lines with elongated morphologies did not show a significant migratory response to SFs.

Conclusions:

  • Scatter factors (SFs) are potent stimulators of vascular endothelial cell migration.
  • Endothelial cell morphology influences responsiveness to SFs, with epithelioid cells being responsive.
  • SFs may play significant roles in physiological processes such as embryonic development and tissue repair.