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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Exon array data analysis using Affymetrix power tools and R statistical software.

Helen E Lockstone1

  • 1Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK. hel23@well.ox.ac.uk

Briefings in Bioinformatics
|April 19, 2011
PubMed
Summary
This summary is machine-generated.

New exon array technology offers a comprehensive analysis of the transcriptome, enabling detailed study of alternative splicing for disease research. This tutorial introduces exon array data analysis, addressing unique challenges and novel tools.

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Related Experiment Videos

Last Updated: Jun 2, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Microarray technology for genome-wide gene expression analysis is established.
  • Standard gene expression data processing and analysis methods are well-developed.
  • The Affymetrix Exon 1.0 ST array offers individual exon-level expression assessment.

Purpose of the Study:

  • To introduce the Affymetrix Exon 1.0 ST array platform.
  • To provide a detailed tutorial for analyzing exon array data.
  • To highlight novel tools and challenges in exon array data analysis.

Main Methods:

  • Utilizing microarray technology for gene expression profiling.
  • Applying established methods for gene expression data analysis.
  • Developing and applying novel tools for exon-level data analysis and alternative splicing studies.

Main Results:

  • Exon arrays enable comprehensive transcriptome analysis.
  • Alternative splicing can be studied in detail using exon array data.
  • Novel analytical approaches are required for exon array data.

Conclusions:

  • Exon arrays provide a more detailed view of the transcriptome compared to traditional microarrays.
  • The study of alternative splicing is facilitated by exon array technology.
  • This work presents a practical guide for researchers using exon arrays.