Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 2, 2026

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
09:04

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

Published on: December 11, 2012

Antennal transcriptome of Manduca sexta.

Ewald Grosse-Wilde1, Linda S Kuebler, Sascha Bucks

  • 1Department for Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany. grosse-wilde@ice.mpg.de

Proceedings of the National Academy of Sciences of the United States of America
|April 19, 2011
PubMed
Summary

Researchers identified key olfactory genes in the tobacco hornworm (Manduca sexta) using transcriptomic and microarray data. This study provides crucial molecular insights into the complex natural behaviors of this important insect model.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A queen odour mediates reproductive suppression in a eusocial mammal.

Nature·2026
Same author

A predator-derived odor specifically triggers avoidance in gregarious mated female Locusta migratoria.

Pest management science·2026
Same author

Effects of elevated ozone on life history traits, olfactory detection, and behavior in Drosophila.

The Science of the total environment·2026
Same author

Oxidizing pollutants can disrupt nestmate recognition in ants.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Carbon dioxide drives oviposition in <i>Helicoverpa armigera</i>.

National science review·2025
Same author

Male-derived PBP4 is essential for sperm competition by mediating sperm motility in moths.

Proceedings of the National Academy of Sciences of the United States of America·2025

Area of Science:

  • Insect olfaction research
  • Molecular biology
  • Genomics and transcriptomics

Background:

  • Understanding insect olfaction is crucial, but research is limited to species with extensive genomic data.
  • Previous studies on Lepidoptera primarily focused on Bombyx mori, which has limited ecological relevance.
  • Manduca sexta, a classical olfactory model with complex natural behaviors, requires detailed molecular data.

Purpose of the Study:

  • To identify members of major olfactory gene families in Manduca sexta.
  • To validate transcriptomic and microarray data using antennal lobe morphology.
  • To compare olfactory gene expression between male and female Manduca sexta antennae.

Main Methods:

  • Transcriptomic analysis to identify olfactory genes.

More Related Videos

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
05:56

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Published on: May 15, 2019

RNAi Trigger Delivery into Anopheles gambiae Pupae
09:58

RNAi Trigger Delivery into Anopheles gambiae Pupae

Published on: March 8, 2016

Related Experiment Videos

Last Updated: Jun 2, 2026

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
09:04

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

Published on: December 11, 2012

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
05:56

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Published on: May 15, 2019

RNAi Trigger Delivery into Anopheles gambiae Pupae
09:58

RNAi Trigger Delivery into Anopheles gambiae Pupae

Published on: March 8, 2016

  • Microarray data to assess gene expression.
  • Correlation of receptor gene expression with antennal lobe morphology.
  • Differential expression analysis between sexes.

    • Main Results:

    • Identification of near-complete transcript sets for key olfactory gene families in Manduca sexta.
    • Validation of gene expression data through morphological correlation.
    • Significant differences in olfactory gene expression observed between male and female antennae.

    Conclusions:

    • This study provides a comprehensive molecular resource for Manduca sexta olfaction.
    • The findings enhance our understanding of olfactory mechanisms in a ecologically relevant insect model.
    • Comparative analysis reveals sex-specific differences in olfactory gene expression, potentially linked to behavior.