Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>CFTR</i> gene delivery to human airway epithelia using parainfluenza virus 5 amplifying virus-like particles.

Molecular therapy. Nucleic acids·2026
Same author

Temporal single-cell atlas of full-length Huntington's disease mouse model defines stage-specific signatures of corticostriatal dysfunction.

Molecular neurodegeneration·2026
Same author

Optimized AAV capsids robustly transduce airway epithelial cells.

bioRxiv : the preprint server for biology·2026
Same author

Neuroepithelial Tumor with AAV Integration after Intracisternal Magna Vector Delivery.

The New England journal of medicine·2026
Same author

Lentiviral-mediated gene complementation to rescue pathogenic ABCA3 variants.

American journal of respiratory cell and molecular biology·2026
Same author

Considerations for early life genetic therapies in cystic fibrosis.

American journal of physiology. Lung cellular and molecular physiology·2026
Same journal

Genetic origins and constraints of evolutionary innovation.

Nature reviews. Genetics·2026
Same journal

Single-cell four-omics with CHARM.

Nature reviews. Genetics·2026
Same journal

Molecular integration of seasonal temperature signals in flowering time control.

Nature reviews. Genetics·2026
Same journal

RBPscan measures protein-RNA interactions in living cells.

Nature reviews. Genetics·2026
Same journal

Revisiting retinal and macular degeneration in the genomics era.

Nature reviews. Genetics·2026
Same journal

How evolution builds three morphs from one genome.

Nature reviews. Genetics·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
12:03

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

Current prospects for RNA interference-based therapies.

Beverly L Davidson1, Paul B McCray

  • 1Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA. beverly-davidson@uiowa.edu

Nature Reviews. Genetics
|April 19, 2011
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) offers a powerful method for gene silencing, with applications in biological research and therapeutic strategies for diseases. Ongoing clinical trials show promise for RNAi-based treatments in humans.

More Related Videos

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

Related Experiment Videos

Last Updated: Jun 2, 2026

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
12:03

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • RNA interference (RNAi) is a conserved biological process that regulates gene expression.
  • It is utilized to reduce the expression of specific proteins, including those involved in disease pathogenesis.
  • RNAi technology has advanced significantly for both research and therapeutic applications.

Purpose of the Study:

  • To provide a comprehensive overview of RNAi-based gene silencing approaches for therapeutic applications.
  • To discuss critical factors in designing RNAi triggers for human use.
  • To review the current landscape of clinical trials employing RNAi.

Main Methods:

  • Delivery of RNA oligonucleotides.
  • Expression of RNAi triggers using viral vectors.
  • Development of targeted gene silencing strategies.

Main Results:

  • Successful demonstration of RNAi in cell culture and in vivo models.
  • Advancement of RNAi-based gene silencing in human clinical trials.
  • Potential for treating fatal disorders and offering alternatives to conventional therapies.

Conclusions:

  • RNAi represents a versatile and potent tool for targeted gene silencing.
  • Careful consideration of RNAi trigger design is crucial for human therapeutic applications.
  • Clinical trials indicate a promising future for RNAi-based therapies in treating various diseases.