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Updated: Jun 2, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
Published on: June 28, 2024
Capillary electrophoresis immunoassays with conjugated quantum dots
Karel Klepárník1, Ivona Voráčová, Marcela Lišková
1Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic. klep@iach.cz
Abstract:
Water-soluble CdTe quantum dots (QDs) and their conjugates with antibodies and antigenes were prepared by optimized procedures for applications in CE immunoassays. The QD size of 3.5 nm, excitation spectrum in the range of 300-500 nm, the maximum wavelength of the emission spectrum at 610 nm, quantum yield of 0.25 and luminescence lifetimes in the range of 3.6-43 ns were determined. The 0.1 M solution of TRIS/TAPS (pH 8.3) was found to be the optimum buffer for the separation of the antiovalbumin-ovalbumin immunocomplex from the free conjugates of QDs.
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