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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
A comprehensive map of the human urinary proteome
Arivusudar Marimuthu1, Robert N O'Meally, Raghothama Chaerkady
1Institute of Bioinformatics, International Technology Park, Bangalore 560066, Karnataka, India.
Journal of Proteome Research
|April 20, 2011
Summary
This study comprehensively catalogs the human urinary proteome using advanced mass spectrometry. Researchers identified 1,823 proteins, including 671 novel urinary proteins, establishing a key reference for disease biomarker discovery.
Area of Science:
- Biochemistry
- Proteomics
- Human Physiology
Background:
- The human urinary proteome offers insights into physiological and pathological states.
- Urinary proteomic studies have potential applications in disease diagnosis.
- Advancements in mass spectrometry enable detailed analysis of low-abundance urine proteins.
Purpose of the Study:
- To conduct a comprehensive analysis of the human urinary proteome in healthy individuals.
- To establish a reference dataset for urinary proteins.
- To identify novel urinary proteins for potential biomarker applications.
Main Methods:
- High-resolution Fourier transform mass spectrometry (Orbitrap) was employed.
- Analysis included both unfractionated and lectin-enriched urine proteins.
- High mass accuracy and resolution settings (MS: 60,000; MS/MS: 15,000) were utilized.
Main Results:
- A total of 1,823 proteins were identified with a false discovery rate below 1%.
- 671 proteins identified in human urine were not previously reported.
- The study provides an extensive catalog of urinary proteins.
Conclusions:
- This comprehensive urinary proteome dataset serves as a valuable reference.
- The findings support future research into urinary biomarkers for disease identification.
- The identified proteins contribute to understanding human physiology and pathology.
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