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Updated: Jun 2, 2026

Whole-cell Patch-clamp Recordings in Brain Slices
Published on: June 15, 2016
Different physiological properties of spontaneous excitatory postsynaptic currents in nucleus accumbens shell neurons
Xiao-Dong Liu1, Zhuo Liu, Qing-Yao Kong
1Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, People's Republic of China.
Abstract:
Studies have demonstrated the changes of the physiological characteristics of nucleus accumbens (NAc) neurons with the postnatal development of rats. In the present study, spontaneous excitatory postsynaptic currents (sEPSCs) were recorded in the slices of NAc shell (NAcS) of adult and juvenile rats. Our results demonstrate that both the average amplitude of sEPSCs and the average frequency of sEPSCs in the NAcS slices of adult rats decreased significantly than that in juvenile rats. The average half width of sEPSCs in the NAcS slices in adult rats increased significantly than that in juvenile rats. The rise time of sEPSCs, the rise 50 time of sEPSCs and the 10-90 rise time of sEPSCs in the NAcS slices increased significantly in adult rats than that in juvenile rats. The decay time of sEPSCs in the NAcS slices also increased significantly in adult rats than that in juvenile rats. The above results strongly indicate that there are marked changes in the electrophysiological properties of single sEPSC in the NAcS slices of juvenile and adult rats.

