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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
A study of histologic and immunophenotypical staining patterns in cutaneous lymphoid hyperplasia
Reuven Bergman1, Khozayma Khamaysi, Ziad Khamaysi
1Department of Dermatology, Rambam Medical Center and the Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, Haifa, Israel. r_bergman@rambam.health.gov.il
Journal of the American Academy of Dermatology
|April 20, 2011
Summary
Histologic and immunohistochemical analysis of cutaneous lymphoid hyperplasia (CLH) identified distinct patterns. This may lead to a new classification system for improved diagnosis of CLH.
Area of Science:
- Dermatopathology
- Immunohistochemistry
- Molecular Pathology
Background:
- Cutaneous lymphoid hyperplasia (CLH) is typically classified by B-cell or T-cell predominance.
- Existing classifications lack detailed histologic and immunophenotypic correlation.
Purpose of the Study:
- To identify specific histologic features and immunohistochemical staining patterns in CLH.
- To establish a basis for a refined histologic classification system for CLH.
Main Methods:
- Clinical, histologic, immunophenotypic, and molecular analyses were performed on 24 CLH cases.
- Cases were categorized based on germinal center formation, histiocytic component, and nonspecific patterns.
Main Results:
- Ten cases showed well-defined lymphoid follicles with germinal centers.
- Six cases had germinal center clusters without defined follicles.
- Four cases exhibited a prominent histiocytic component; three were nonspecific.
- Most cases lacked clonal gene rearrangements, with exceptions in uneventful long-term follow-ups.
Conclusions:
- A limited number of cases were studied.
- A classification system integrating histologic, immunophenotypic, clinical, and molecular data may improve CLH diagnosis.
