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Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

Concordance between thyroglobulin antibody assays.

Kevin P Taylor¹, Damon Parkington, Sonia Bradbury

¹Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, UK.

Annals of Clinical Biochemistry

|April 20, 2011

Summary

Antithyroglobulin antibody assays show significant variance, impacting thyroid cancer monitoring. Adjusting cut-offs improved concordance, but current methods remain unreliable for patients post-thyroidectomy.

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Area of Science:

Clinical chemistry
Immunodiagnostics
Endocrinology

Background:

Antithyroglobulin antibodies frequently interfere with serum thyroglobulin immunoassays.
Guidelines recommend concurrent measurement of antithyroglobulin antibodies and thyroglobulin for thyroid cancer patient monitoring.
Concerns exist regarding the concordance of various antithyroglobulin assays, despite an international reference preparation.

Purpose of the Study:

To compare the concordance of four commonly used antithyroglobulin assays in UK laboratories.
To evaluate the reliability of these assays in monitoring thyroid cancer patients.

Main Methods:

Four antithyroglobulin assays (Siemens Immulite®, Brahms GmbH, PerkinElmer AutoDELFIA, Siemens ADVIA Centaur®) were analyzed.
A cohort of 145 thyroid cancer patients was studied.
Recovery of exogenous thyroglobulin was assessed using the Brahms Tg-plus assay.

Main Results:

Initial concordance between assays was 74% based on manufacturer reference data.
Adjusting assay cut-offs to maximize agreement increased concordance to 90%.
The Brahms Tg-plus assay was neither specific nor sensitive for detecting positive antibody results.

Conclusions:

Current antithyroglobulin assays exhibit unacceptable variability, even with an international reference preparation.
The findings highlight the need for improved standardization and validation of antithyroglobulin assays.
Clinical decisions based on thyroglobulin measurements may be compromised by assay discordance.