Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Abuse Potential and Neurotoxic Effects of the Synthetic Cannabinoid 4F-ABUTINACA Self-Administration in Adult Male Rats.

Addiction biology·2026
Same author

Improved positive predictive value of non-invasive prenatal testing through integration with second-trimester ultrasound soft markers for fetal chromosomal abnormalities: a retrospective cohort study.

Frontiers in endocrinology·2026
Same author

Molecular characteristics of Chinese colorectal cancer patients with microsatellite instability.

Translational gastroenterology and hepatology·2026
Same author

Reinforcing and discriminative stimulus effects of 4-fluorobutyrfentanyl, 4-fluoroisobutyrfentanyl, and isobutyrfentanyl in male rats.

European journal of pharmacology·2026
Same author

Contextualizing contemporary seasonality variations in East Asian tropical cyclone landfalls with a multi-century historical baseline.

NPJ climate and atmospheric science·2026
Same author

Determination of short-chain chlorinated paraffins in marine sediment by automated online solid phase extraction coupled to liquid chromatography-high-resolution mass spectrometry.

Analytica chimica acta·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Single-shot full-field reflection phase microscopy.

Zahid Yaqoob1, Toyohiko Yamauchi, Wonshik Choi

  • 1G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

Optics Express
|April 20, 2011
PubMed
Summary
This summary is machine-generated.

We developed a novel microscope combining low-coherence interferometry and digital holographic microscopy (DHM) for sensitive, depth-selective imaging of live cells. This technology enables nanometer-scale measurements of cellular membrane dynamics.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Related Experiment Videos

Last Updated: Jun 2, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Cell Biology

Background:

  • Accurate measurement of cellular dynamics is crucial for understanding cell function.
  • Existing microscopy techniques face limitations in sensitivity and depth selectivity for live-cell imaging.

Purpose of the Study:

  • To develop a full-field reflection phase microscope combining low-coherence interferometry and digital holographic microscopy (DHM).
  • To achieve highly sensitive, single-shot, and depth-selective imaging of cellular dynamics.
  • To enable nanometer-scale measurements of membrane dynamics in live cells.

Main Methods:

  • The study employed a reflection-based digital holographic microscopy setup.
  • Low-coherence interferometry was integrated for depth-selective measurements.
  • A diffraction grating in the reference arm ensured uniform contrast with a low-coherence light source.

Main Results:

  • The developed microscope provides highly sensitive and single-shot imaging of cellular dynamics.
  • Depth-selective measurements were achieved using a low-coherence light source.
  • The instrument demonstrated a path-length sensitivity of approximately 21 picometers/Hz.

Conclusions:

  • The novel microscope is suitable for nanometer-scale full-field measurements of membrane dynamics in live cells.
  • The combination of techniques offers enhanced capabilities for live-cell imaging and biophysical studies.