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Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...

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Related Experiment Video

Updated: Jun 2, 2026

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
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Published on: August 6, 2018

Picosecond pulse amplification in isotopic CO2 active medium.

Mikhail N Polyanskiy1, Igor V Pogorelsky, Vitaly Yakimenko

  • 1Brookhaven National Laboratory, Bldg. 820M, Upton, NY 11973, USA. polyanskiy@bnl.gov

Optics Express
|April 20, 2011
PubMed
Summary

Researchers developed a high-pressure carbon dioxide (CO(2)) laser amplifier using oxygen-18. This innovation produced a 1 TW peak power pulse without spectral splitting, overcoming a common limitation in CO(2) laser amplification.

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Last Updated: Jun 2, 2026

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
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Published on: August 6, 2018

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Area of Science:

  • Laser physics
  • Quantum optics
  • Isotope enrichment

Background:

  • Carbon dioxide (CO(2)) lasers are crucial for various scientific applications.
  • Spectral modulation due to rotational structure limits peak power in CO(2) laser amplifiers.
  • Isotopic enrichment is a potential method to alter laser properties.

Purpose of the Study:

  • To investigate the effect of oxygen-18 enrichment on a high-pressure CO(2) laser amplifier.
  • To achieve high peak power pulses without spectral splitting.
  • To overcome limitations imposed by the CO(2) amplification band's rotational structure.

Main Methods:

  • Utilized a high-pressure carbon dioxide laser amplifier.
  • Enriched the amplifier medium with the oxygen-18 isotope.
  • Characterized the output pulse properties, including duration, wavelength, and peak power.

Main Results:

  • Successfully produced a 5-picosecond (ps) pulse at a 10-micrometer (µm) wavelength.
  • Achieved a peak power of 1 terawatt (TW).
  • Demonstrated pulse generation without the typical spectral splitting caused by rotational modulation.

Conclusions:

  • Oxygen-18 enrichment in CO(2) laser amplifiers can mitigate spectral modulation.
  • This technique enables the generation of high-energy, high-power laser pulses.
  • Opens new possibilities for advanced laser applications requiring high peak power.