Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Permanent pacemaker rate following Commando and Hemi-Commando procedures: a systematic review and meta-analysis.

Frontiers in cardiovascular medicine·2026
Same author

Mutations in nucleoporins and their roles in human diseases: Integrating evidence on specific NUP functions.

European journal of cell biology·2026
Same author

NUDT21 regulates 3'UTR dynamics in epididymal principal cells to preserve sperm integrity.

Development (Cambridge, England)·2026
Same author

Safety and effectiveness of etonogestrel implant in Chinese women requiring contraception: a multicenter, prospective, observational study.

Archives of gynecology and obstetrics·2026
Same author

Disinfection Byproducts, Oxidative Stress, and Sleep Quality among Healthy Chinese Men.

Environment & health (Washington, D.C.)·2026
Same author

A de novo C-terminal truncation mutation in NUP205 as a key factor in premature ovarian insufficiency.

Human reproduction (Oxford, England)·2026

Related Experiment Video

Updated: Jun 2, 2026

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response
13:04

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

Published on: May 7, 2012

CR16 forms a complex with N-WASP in human testes.

Wenpei Xiang1, Zina Wen, Wenjuan Pang

  • 1Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.

Cell and Tissue Research
|April 20, 2011
PubMed
Summary
This summary is machine-generated.

Reduced levels of corticosteroids and regional expression 16 (CR16) and neural Wiscott-Aldrich syndrome protein (N-WASP) in testes are linked to idiopathic azoospermia. These proteins co-localize in Sertoli cell junctions, suggesting a role in male infertility.

More Related Videos

Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes
08:37

Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

Published on: August 27, 2020

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Related Experiment Videos

Last Updated: Jun 2, 2026

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response
13:04

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

Published on: May 7, 2012

Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes
08:37

Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

Published on: August 27, 2020

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Area of Science:

  • Reproductive Biology
  • Molecular Endocrinology
  • Male Infertility Research

Background:

  • Idiopathic azoospermia affects sperm production, with underlying molecular mechanisms often unclear.
  • Corticosteroids and regional expression 16 (CR16) and neural Wiscott-Aldrich syndrome protein (N-WASP) are proteins potentially involved in testicular function.

Purpose of the Study:

  • To investigate the expression and localization of CR16 and N-WASP in the testes of men with idiopathic azoospermia.
  • To determine if CR16 and N-WASP play a role in the pathogenesis of idiopathic azoospermia.

Main Methods:

  • Immunohistochemistry and Western blot to detect CR16 and N-WASP protein expression.
  • Reverse transcription-polymerase chain reaction (RT-PCR) to quantify CR16 and N-WASP mRNA levels.
  • Double-staining immunofluorescence and laser scanning confocal microscopy (LSCM) for protein co-localization.

Main Results:

  • CR16 and N-WASP proteins were found in Sertoli cells, specifically at Sertoli cell-spermatogenic cell junctions (SspJs).
  • Significantly decreased CR16 and N-WASP mRNA levels were observed in men with idiopathic azoospermia compared to healthy controls.
  • CR16 and N-WASP proteins co-localized at the SspJs, forming a potential CR16/N-WASP complex.

Conclusions:

  • Reduced expression of CR16 and N-WASP in testicular tissue is associated with idiopathic azoospermia.
  • The co-localization of CR16 and N-WASP as a complex at SspJs suggests their involvement in maintaining normal spermatogenesis.
  • These findings implicate CR16 and N-WASP in the pathogenesis of male infertility due to azoospermia.