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Quantification of specific E. coli in gut mucosa from Crohn's disease patients
Stina Rikke Jensen1, Lisbeth Nielsen Fink, Carsten Struve
1Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. SJEN0236@heh.regionh.dk
Abstract:
We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
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