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Related Concept Videos

Golgi Apparatus01:49

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Transport Across the Golgi01:26

Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
Golgi Matrix Proteins01:12

Golgi Matrix Proteins

Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

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Related Experiment Video

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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

Golgi positioning.

Smita Yadav1, Adam D Linstedt

  • 1Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.

Cold Spring Harbor Perspectives in Biology
|April 21, 2011
PubMed
Summary
This summary is machine-generated.

Cytoplasmic dynein motors move cargo carriers inward to the Golgi apparatus and position it near the cell center. Identifying the Golgi receptor for dynein is crucial for understanding this process.

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Area of Science:

  • Cell Biology
  • Molecular Motors
  • Organelle Positioning

Background:

  • The Golgi apparatus is centrally located near the microtubule-organizing center in mammalian cells.
  • Secretory cargo transport involves inward movement to the Golgi and outward transport to the plasma membrane.
  • Cytoplasmic dynein motors are key for inward cargo movement and Golgi positioning along microtubules.

Purpose of the Study:

  • To investigate the role of cytoplasmic dynein in Golgi positioning and intracellular transport.
  • To identify the specific Golgi receptor for dynein, which remains elusive.
  • To understand the regulation of dynein-mediated centripetal motility.

Main Methods:

  • The study focuses on the known functions of cytoplasmic dynein and Golgi apparatus positioning.
  • It highlights the lack of identified Golgi receptors for dynein.
  • The research context involves studying motor protein-microtubule interactions and organelle dynamics.

Main Results:

  • Cytoplasmic dynein motors mediate inward movement of cargo carriers towards the centrosome.
  • Dynein motors are essential for maintaining the pericentrosomal position of the Golgi apparatus.
  • The precise regulators of dynein's centripetal motility and Golgi receptor are yet to be identified.

Conclusions:

  • Identifying the Golgi receptor for dynein is critical for understanding membrane-motor-microtubule attachment and motor recycling.
  • Dynamic Golgi positioning is regulated during key cellular processes like mitosis and cell migration.
  • Proper Golgi positioning is vital for directed secretion and maintaining cell polarity.