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Mechanical Manipulation of Neurons to Control Axonal Development
Published on: April 10, 2011
Mechanical manipulation of neurons to control axonal development
Phillip Lamoureux1, Steven Heidemann, Kyle E Miller
1Department of Zoology, Michigan State University, East Lansing, Michigan, USA.
Journal of Visualized Experiments : Jove
|April 21, 2011
Summary
Calibrated glass micro-fibers enable precise measurement and application of forces for cell manipulation and neuronal axon growth. This flexible technique offers real-time force visualization for diverse cellular investigations.
Area of Science:
- Biophysics
- Cell Biology
- Neuroscience
Background:
- Cellular processes like axonal growth are influenced by mechanical forces.
- Precise measurement and application of forces are crucial for understanding cell behavior.
Purpose of the Study:
- To describe a methodology for cell manipulation using calibrated glass micro-fibers.
- To enable measurement and application of forces in the 10-1000 μdyne range for cellular studies.
Main Methods:
- Fabrication, calibration, and utilization of glass micro-needles.
- Observation of Hookean bending for force measurement.
- Direct application of forces to cultured cells.
Main Results:
- Demonstrated capability for measuring and applying forces (10-1000 μdyne) to cells.
- Successful manipulation of neuronal axon extension, growth, and retraction.
- Versatile application across different cell types.
Conclusions:
- The described method provides a flexible and effective tool for cellular force manipulation.
- Offers continuous force visualization and direct intervention in cellular events.
- Facilitates mechanical measurements and regulation of cell behavior, including axonal dynamics.
