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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 2, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Secretome profiling with antibody microarrays.

Shakhawan Abdulrahman Mustafa¹, Jörg D Hoheisel, Mohamed Saiel Saeed Alhamdani

¹Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.

Molecular Biosystems

|April 21, 2011

Summary

Antibody microarrays offer a powerful new method for analyzing the secretome, which includes secreted proteins. This technology enhances our understanding of cellular interactions and disease biomarker discovery.

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Last Updated: Jun 2, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

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Area of Science:

Proteomics and human secretome analysis.

Background:

Advances in human genome sequencing have shifted focus to understanding protein functions.
Secreted proteins play crucial roles in intercellular communication and disease biomarker discovery.
Proteomic analysis of the secretome is essential for biological function elucidation.

Purpose of the Study:

To review recent applications of antibody microarrays in secretome analysis.
To highlight the potential of antibody microarrays for advancing proteomics.
To discuss the future prospects of this technology in secretome research.

Main Methods:

Utilizing antibody microarrays for high-sensitivity proteomic analysis.
Assaying variations in protein amount, structure, and activity simultaneously.
Leveraging novel platform technology for secretome exploration.

Main Results:

Antibody microarrays demonstrate simplicity, robust performance, and single-molecule detection sensitivity.
This technology improves analytical capabilities in proteomics.
Enables the production of more informative and reliable secretome data.

Conclusions:

Antibody microarrays are a promising tool for secretome analysis.
The technology offers unique opportunities for understanding cellular interactions and biomarker discovery.
Future applications are expected to significantly advance the field of proteomics.