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Related Experiment Video

Updated: Jun 2, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

Lectin microarray profiling of metastatic breast cancers.

Simon A Fry1, Babak Afrough, Hannah J Lomax-Browne

  • 1Breast Cancer Research Group, EGA Institute for Women's Health, University College London, London WC1E 6BT, UK. s.fry@wibr.ucl.ac.uk

Glycobiology
|April 22, 2011
PubMed
Summary

This study introduces a novel lectin microarray for analyzing breast cancer glycosylation. The technique efficiently detects cancer-associated glycan changes in tumors, serum, and urine, offering potential for biomarker discovery.

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Area of Science:

  • Biochemistry
  • Oncology
  • Glycomics

Background:

  • Altered protein glycosylation is a hallmark of cancer cells.
  • Specific glycan structures are linked to cancer types, but comprehensive tumor glycome data is limited.
  • Existing techniques for glycosylation analysis hinder biomarker discovery.

Purpose of the Study:

  • To develop and validate a lectin microarray for comprehensive glycomic profiling of breast cancer.
  • To assess the potential of this method for identifying disease-associated glycosylation alterations.
  • To evaluate the utility of the technique for biomarker discovery in metastatic breast cancer.

Main Methods:

  • Utilized lectin microarrays with 45 lectins targeting N- and O-linked glycans.
  • Employed evanescent-field activated fluorescent detection for glycomic analysis.

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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

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Last Updated: Jun 2, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

  • Analyzed primary breast tumors, serum, and urine from patients with metastatic breast cancer.

    • Main Results:

    • Detected significant glycosylation alterations in metastatic breast cancer using minimal sample amounts (50 µm tumor section or <1 µL biofluid).
    • Lectin-binding patterns accurately inferred disease-associated glycosylation changes.
    • Demonstrated the high-throughput, sensitive, and simple nature of the assay.

    Conclusions:

    • Lectin microarray analysis is a valuable tool for discovery-phase glycomic profiling.
    • The method allows simultaneous analysis of N- and O-linked glycosylation without protein deglycosylation.
    • This approach facilitates the identification of novel cancer biomarkers from minimal clinical samples.