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Establishment of an IL-2-dependent, antigen nonspecific chicken T-cell line
1Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, Nogent-sur-Marne.
Abstract:
In this article, we describe a-chicken T helper-activated cell line, III-C5, which is non-antigen-specific but IL-2-dependent. By virtue of its absolute requirement for IL-2, the III-C5 cell line is a useful tool for quantifying IL-2 production by any chicken-activated T lymphocytes in culture. The III-C5 line will be used to quantify IL-2 production in mixed lymphocyte reaction in vitro, in order to study the functional activity of T lymphocytes from avian chimeras constructed in our laboratory and particularly for studying their state of tolerance.
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