Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 8, 2025

Systemic Treatment for Postnatal, Juvenile, and Runted Adult Mice by Retrobulbar Sinus Injection
02:39

Systemic Treatment for Postnatal, Juvenile, and Runted Adult Mice by Retrobulbar Sinus Injection

Published on: May 17, 2024

1.6K

Retro-orbital injections in mice.

Tal Yardeni1, Michael Eckhaus, H Douglas Morris

  • 1Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Lab Animal
|April 22, 2011
PubMed
Summary

Retro-orbital injection offers a viable alternative for administering compounds in adult and neonatal mice. This venous sinus injection technique is simpler than tail vein injections for non-tumorigenic substances.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondria across the globe: diverse voices, shared energy.

Trends in endocrinology and metabolism: TEM·2026
Same author

A mouse model of free sialic acid storage disorder: Hypomyelinating leukodystrophy and Purkinje cell degeneration.

Disease models & mechanisms·2026
Same author

Phase 1 Study of Oral N-Acetylmannosamine in Primary Podocytopathies.

Kidney international reports·2026
Same author

Bioenergetic Signatures of DLD Deficiency: Dissecting PDHc- and α-KGDHc-Linked Defects.

Antioxidants (Basel, Switzerland)·2026
Same author

Molecular and biochemical insights into dysregulation of glycosphingolipid metabolism in a mouse model of lysosomal free sialic acid storage disorder.

Experimental neurology·2026
Same author

Deep Learning-Based Denoising for High-Resolution Carotid Vessel Wall MRI Using Standard Neurovascular Coils.

Magnetic resonance in medicine·2025

Area of Science:

  • Veterinary Medicine
  • Anesthesiology
  • Pharmacology

Background:

  • Intravenous vascular access in mice, particularly neonates, presents significant technical challenges.
  • Existing methods like tail vein injection can be difficult and stressful for the animals.

Purpose of the Study:

  • To describe and evaluate the retro-orbital venous sinus injection technique for compound administration in adult and neonatal mice.
  • To present this method as a practical alternative to tail vein injections.

Main Methods:

  • Detailed description of the retro-orbital injection procedure in adult and neonatal mouse models.
  • Assessment of the technique's feasibility and volume capacity for compound delivery.

Main Results:

More Related Videos

A Neonatal Rodent Model of Retroorbital Vein Injection
04:39

A Neonatal Rodent Model of Retroorbital Vein Injection

Published on: February 23, 2024

7.2K
Retro-orbital Injection in Adult Zebrafish
04:50

Retro-orbital Injection in Adult Zebrafish

Published on: December 7, 2009

25.2K

Related Experiment Videos

Last Updated: Oct 8, 2025

Systemic Treatment for Postnatal, Juvenile, and Runted Adult Mice by Retrobulbar Sinus Injection
02:39

Systemic Treatment for Postnatal, Juvenile, and Runted Adult Mice by Retrobulbar Sinus Injection

Published on: May 17, 2024

1.6K
A Neonatal Rodent Model of Retroorbital Vein Injection
04:39

A Neonatal Rodent Model of Retroorbital Vein Injection

Published on: February 23, 2024

7.2K
Retro-orbital Injection in Adult Zebrafish
04:50

Retro-orbital Injection in Adult Zebrafish

Published on: December 7, 2009

25.2K
  • The retro-orbital injection technique was successfully employed in adult mice for volumes up to 150 microliters without complications.
  • While more challenging in neonates, the method reliably delivered volumes up to 10 microliters.

Conclusions:

  • Retro-orbital venous sinus injection is an effective and less invasive method for administering non-tumorigenic compounds in mice.
  • This technique provides a valuable alternative for researchers needing to deliver substances intravenously in both adult and young mouse models.