Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Balloon valvuloplasty in a dog with congenital bicuspid aortic valve and supravalvar aortic stenosis (atypical Shone's complex).

Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2019
Same author

Central nervous system lymphoma in 18 dogs (2001 to 2015).

The Journal of small animal practice·2018
Same author

Modulation of mitochondrial function by the microbiome metabolite propionic acid in autism and control cell lines.

Translational psychiatry·2016
Same author

Clinical Features and Magnetic Resonance Imaging Findings in 7 Dogs with Central Nervous System Aspergillosis.

Journal of veterinary internal medicine·2015
Same author

The influence of maternal energy status during mid-gestation on beef offspring tenderness, muscle characteristics, and gene expression.

Meat science·2015
Same author

The influence of maternal energy status during midgestation on beef offspring carcass characteristics and meat quality.

Journal of animal science·2014

Related Experiment Video

Updated: Jun 2, 2026

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Subcellular features revealed on unfixed rat brain sections by phase imaging.

H-Y Nie1, A R Taylor, W M Lau

  • 1Surface Science Western, The University of Western Ontario, 999 Collip Circle, London, Ontario N6G 0J3, Canada. hnie@uwo.ca

The Analyst
|April 22, 2011
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy phase imaging reveals biological structures on rat brain sections by mapping mechanical properties. This technique complements traditional microscopy for visualizing native tissue states.

More Related Videos

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
09:59

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

Published on: February 12, 2014

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals
06:25

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals

Published on: January 19, 2024

Related Experiment Videos

Last Updated: Jun 2, 2026

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
09:59

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

Published on: February 12, 2014

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals
06:25

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals

Published on: January 19, 2024

Area of Science:

  • Biophysics
  • Materials Science
  • Neuroscience

Background:

  • Atomic force microscopy (AFM) topographic imaging alone offers limited information for detailing biological structures in sectioned tissues.
  • Traditional imaging methods like optical and electron microscopy have limitations in visualizing the native state of biological samples.

Purpose of the Study:

  • To demonstrate the utility of amplitude-modulation AFM phase imaging for mapping structures on unfixed biological tissues.
  • To highlight phase imaging as a complementary technique for visualizing mechanical properties of biological structures.

Main Methods:

  • Utilizing amplitude-modulation atomic force microscopy (AFM) on unfixed rat brain sections.
  • Acquiring phase images to capture variations in mechanical properties across the tissue surface.

Main Results:

  • Phase imaging effectively mapped structures on the surface of unfixed rat brain sections.
  • Contrast in phase images directly correlated with differences in the mechanical properties of biological structures.

Conclusions:

  • Amplitude-modulation AFM phase imaging is a powerful tool for visualizing biological structures based on their mechanical properties.
  • This method provides complementary information to traditional microscopy, enabling visualization of biological samples in a more native state.