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Updated: Jun 2, 2026
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In vitro Functional Characterization of Mouse Colorectal Afferent Endings
Published on: January 21, 2015
Colitis decreases mechanosensitive K2P channel expression and function in mouse colon sensory neurons
1Center for Pain Research, Department of Anesthesiology, University of Pittsburgh, Pennsylvania, USA. laj@upmc.edu
Summary
Mechanosensitive K(2P) channels in colon sensory neurons are reduced by inflammation. This decrease in TREK-1, TREK-2, and TRAAK channel expression and activity contributes to colon hypersensitivity in inflammatory bowel conditions.
Area of Science:
- Neuroscience
- Gastroenterology
- Ion Channel Physiology
Background:
- Mechanosensitive K(2P) channels (TREK-1, TREK-2, TRAAK) attenuate mechanotransduction.
- Colon inflammation causes hypersensitivity, suggesting altered channel function.
Purpose of the Study:
- Investigate the role of K(2P) channels in colon sensory neurons.
- Determine if colon inflammation affects these channels in dorsal root ganglion (DRG) neurons.
Main Methods:
- Studied K(2P) channel expression (mRNA and electrophysiology) in mouse colon DRG neurons (TL and LS).
- Induced colon inflammation using trinitrobenzene sulfonic acid.
- Assessed changes in channel expression and activity post-inflammation.
Main Results:
- Most colon DRG neurons express TREK-1, TREK-2, and TRAAK channels.
- Inflammation significantly decreased TREK-1 mRNA and TREK-2 channel activity in LS DRG neurons.
- Osmotic stretch response was reduced in LS colon DRG neurons after inflammation.
Conclusions:
- The majority of DRG neurons innervating the colon express mechanosensitive K(2P) channels.
- Reduced expression and activity of these channels contribute to colon hypersensitivity during inflammation.
- Findings suggest K(2P) channels are key players in inflammatory bowel-related visceral pain.
