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Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

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Related Experiment Video

Updated: Jun 2, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

Mitochondrial structure and function are disrupted by standard isolation methods.

Martin Picard1, Tanja Taivassalo, Darmyn Ritchie

  • 1Department of Kinesiology, McGill University, Montreal, Quebec, Canada.

Plos One
|April 23, 2011
PubMed
Summary
This summary is machine-generated.

Studying skeletal muscle mitochondrial function using isolated mitochondria alters organelle structure and function. Permeabilized myofibers better preserve mitochondrial integrity for accurate in vivo assessments.

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Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
08:11

Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

Published on: February 10, 2022

Area of Science:

  • Mitochondrial physiology
  • Cellular biology
  • Skeletal muscle research

Background:

  • Mitochondria are vital for cellular energy production, calcium handling, and apoptosis.
  • Studying mitochondrial function in skeletal muscle typically uses isolated mitochondria or permeabilized myofibers.
  • Isolated mitochondria are commonly used but may not reflect in vivo conditions.

Purpose of the Study:

  • To directly compare mitochondrial function measurements between isolated mitochondria and permeabilized myofibers.
  • To assess the impact of mitochondrial isolation on morphology, calcium handling, respiration, and oxidative stress.
  • To evaluate alterations in electron transport chain protein stoichiometry due to isolation.

Main Methods:

  • Comparison of mitochondrial function in isolated rat gastrocnemius mitochondria versus permeabilized myofibers.
  • Assessment of mitochondrial morphology, permeability transition pore sensitivity, respiration, and hydrogen peroxide production.
  • Analysis of electron transport chain protein stoichiometry.

Main Results:

  • Mitochondrial isolation induced fragmented morphology and increased sensitivity of the permeability transition pore to calcium.
  • Respiration patterns were altered differently under various conditions, and hydrogen peroxide production significantly increased.
  • Electron transport chain protein stoichiometry was markedly changed by isolation.
  • Isolated mitochondria exhibited functional characteristics fundamentally different from those in intact permeabilized myofibers.

Conclusions:

  • Mitochondrial isolation significantly alters mitochondrial structure and function compared to permeabilized myofibers.
  • Results suggest isolated mitochondria may not accurately represent in vivo mitochondrial function.
  • Preserving mitochondrial structure in preparations like permeabilized myofibers is crucial for reliable functional studies.